Measurement of the Sieve Tube Membrane Potential
نویسندگان
چکیده
منابع مشابه
Measurement of the sieve tube membrane potential.
A procedure is described for the measurement of the sieve tube membrane potential in the phloem of bark strips from Salix exigua Nutt. Measurements were made by inserting a measuring microelectrode into sap exuding from severed stylets of the willow aphid, Tuberolachnus salignus. Data taken from 20 bark strips gave an average potential of -155 +/- 9 millivolts. Evidence is presented for an elec...
متن کاملDirect measurement of sieve tube turgor pressure using severed aphid stylets.
Turgor pressure in individual sieve tubes was measured directly by gluing capillary micromanometers over exuding aphid stylets with cyanoac-rylate adhesive. Pressures of up to 10 bars were measured in sieve tubes of Salix babylonica, with an estimated accuracy of +/- 0.3 bars or better.For comparison with the direct measurements of sieve tube turgor, calculated values of turgor pressure were al...
متن کاملTurnover of soluble proteins in the wheat sieve tube.
Although the enucleate conducting cells of the phloem are incapable of protein synthesis, phloem exudates characteristically contain low concentrations of soluble proteins. The role of these proteins and their movement into and out of the sieve tubes poses important questions for phloem physiology and for cell-to-cell protein movement via plasmodesmata. The occurrence of protein turnover in sie...
متن کاملThe measurement of membrane potential using optical indicators.
I n t r o d u c t i o n Biological membranes are d i sc re te organelles of finite thickness which separate cells from each other and partition the i n t r a c e l l u l a r space of eukaryotes into a number of separate, aqueous compartments. Many of the membranes actively maintain a significant, e l e c t r o s t a t i c po t en t i a l d i f f e r e n c e , the membrane potential, between the...
متن کاملAphid salivary proteases are capable of degrading sieve-tube proteins.
Sieve tubes serve as transport conduits for photo-assimilates and other resources in angiosperms and are profitable targets for piercing-sucking insects such as aphids. Sieve-tube sap also contains significant amounts of proteins with diverse functions, for example in signalling, metabolism, and defence. The identification of salivary proteases in Acyrthosiphon pisum led to the hypothesis that ...
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ژورنال
عنوان ژورنال: Plant Physiology
سال: 1981
ISSN: 0032-0889,1532-2548
DOI: 10.1104/pp.67.4.845